The phage T4 restriction endoribonuclease RegB: a cyclizing enzyme that requires two histidines to be fully active.

@article{Sada2003ThePT,
  title={The phage T4 restriction endoribonuclease RegB: a cyclizing enzyme that requires two histidines to be fully active.},
  author={Fakhri Sa{\"i}da and Marc Uzan and François Bontems},
  journal={Nucleic acids research},
  year={2003},
  volume={31 11},
  pages={2751-8}
}
The regB gene, from the bacteriophage T4, codes for an endoribonuclease that controls the expression of a number of phage early genes. The RegB protein cleaves its mRNA substrates with an almost absolute specificity in the middle of the tertranucleotide GGAG, making it a unique well-defined restriction endoribonuclease. This striking protein has no homology to any known RNase and its catalytic mechanism has never been investigated. Here, we show, using 31P nuclear magnetic resonance (NMR), that… CONTINUE READING