The pattern of cytokine gene expression in freshly excised human metastatic melanoma suggests a state of reversible anergy of tumor-infiltrating lymphocytes.

@article{Lscher1994ThePO,
  title={The pattern of cytokine gene expression in freshly excised human metastatic melanoma suggests a state of reversible anergy of tumor-infiltrating lymphocytes.},
  author={Urs L{\"u}scher and Luis Filgueira and Antonio Jureti{\'c} and Markus Zuber and N. J. L{\"u}scher and Michael M. G. Heberer and Giulio Cesare Spagnoli},
  journal={International journal of cancer},
  year={1994},
  volume={57 4},
  pages={612-9}
}
Expression of an extended panel of cytokine genes was investigated by reverse polymerase chain reaction (PCR) in 10 freshly excised melanoma metastases infiltrated by lymphocytes (TIL). cDNA encoding for CD3-delta and tyrosinase could be amplified in all samples, confirming the presence of T lymphocytes and melanoma cells. Cytokine genes possibly transcribed by both cell types, such as GM-CSF, IL-6 and IL-10 could be amplified from 5, 2 and 2 samples respectively. In contrast, IL-1 beta and TNF… CONTINUE READING