The past, present and future of cell-free protein synthesis.

  title={The past, present and future of cell-free protein synthesis.},
  author={Federico Katzen and Geoffrey Chang and Wieslaw Kudlicki},
  journal={Trends in biotechnology},
  volume={23 3},
Recent technical advances have revitalized cell-free expression systems to meet the increasing demands for protein synthesis. Cell-free systems offer several advantages over traditional cell-based expression methods, including the easy modification of reaction conditions to favor protein folding, decreased sensitivity to product toxicity and suitability for high-throughput strategies because of reduced reaction volumes and process time. Moreover, improvements in translation efficiency have… CONTINUE READING

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