The particulate methane monooxygenase gene pmoA and its use as a functional gene probe for methanotrophs.

@article{McDonald1997ThePM,
  title={The particulate methane monooxygenase gene pmoA and its use as a functional gene probe for methanotrophs.},
  author={Ian R. McDonald and John Colin Murrell},
  journal={FEMS microbiology letters},
  year={1997},
  volume={156 2},
  pages={205-10}
}
The particulate methane monooxygenase gene pmoA, encoding the 27 kDa polypeptide of the membrane-bound particulate methane monooxygenase, was amplified by PCR from DNA isolated from a blanket peat bog and from enrichment cultures established, from the same environment, using methane as sole carbon and energy source. The resulting 525 bp PCR products were cloned and a representative number of clones were sequenced. Phylogenetic analysis of the derived amino acid sequences of the pmoA clones… CONTINUE READING
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