The particle SSV1 from the extremely thermophilic archaeon Sulfolobus is a virus: demonstration of infectivity and of transfection with viral DNA.

@article{Schleper1992ThePS,
  title={The particle SSV1 from the extremely thermophilic archaeon Sulfolobus is a virus: demonstration of infectivity and of transfection with viral DNA.},
  author={C. Schleper and K. Kubo and W. Zillig},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89},
  pages={7645 - 7649}
}
  • C. Schleper, K. Kubo, W. Zillig
  • Published 1992
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
The lemon-shaped "virus-like" particle SSV1 produced by the thermophilic archaeon Sulfolobus shibatae has not previously been observed to infect any host. Using a plaque assay suitable for the extreme growth conditions of this archaeon, we have shown infection of Sulfolobus solfataricus by SSV1. Upon infection, the viral genome was always found integrated into a tRNA gene of the host chromosome, a situation similar to that in S. shibatae, proving that site-specific integration is involved in… Expand
Genetic requirements for the function of the archaeal virus SSV1 in Sulfolobus solfataricus: construction and testing of viral shuttle vectors.
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Molecular Cloning: A Laboratory Manual
TLDR
The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Expand