The paradoxical effect of acetaminophen on CYP3A4 activity and content in transfected HepG2 cells.

@article{Feierman2002ThePE,
  title={The paradoxical effect of acetaminophen on CYP3A4 activity and content in transfected HepG2 cells.},
  author={Dennis E Feierman and Zoya Melnikov and Jie Zhang},
  journal={Archives of biochemistry and biophysics},
  year={2002},
  volume={398 1},
  pages={
          109-17
        }
}
HepG2 cell lines that constitutively and stably express human CYP3A4 were constructed in order to study enzyme interactions with CYP3A4 as the only P450 present. CYP3A4 activity and content were assessed by the metabolism of fentanyl, a CYP3A substrate, and Western blots. Northern blots were used to examine the effects of acetaminophen (APAP) on CYP3A4-mRNA. The HepG2 cell lines' CYP3A4 activity was stable over time. High concentrations of APAP inhibited CYP3A4 activity. At lower concentrations… CONTINUE READING
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