The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

@article{Vieira1982ThePP,
  title={The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.},
  author={J J Vieira and Joachim Messing},
  journal={Gene},
  year={1982},
  volume={19 3},
  pages={259-68}
}
A series of plasmid vectors containing the multiple cloning site (MCS7) of M13mp7 has been constructed. In one of these vectors a kanamycin-resistance marker has been inserted into the center of the symmetrical MCS7 to yield a restriction-site-mobilizing element (RSM). The drug-resistance marker can be cleaved out of this vector with any of the restriction enzymes that recognize a site of the flanking sequences of the RSM to generate an RSM with either various sticky ends or blunt ends. These… CONTINUE READING
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