The p300/CBP acetyltransferases function as transcriptional coactivators of beta-catenin in vertebrates.

Abstract

Wnt growth factors regulate a variety of developmental processes by altering specific gene expression patterns. In vertebrates beta-catenin acts as transcriptional activator, which is needed to overcome target gene repression by Groucho/TLE proteins, and to permit promoter activation as the final consequence of Wnt signaling. However, the molecular mechanisms of transcriptional activation by beta-catenin are only poorly understood. Here we demonstrate that the closely related acetyltransferases p300 and CBP potentiate beta-catenin-mediated activation of the siamois promoter, a known Wnt target. beta-catenin and p300 also synergize to stimulate a synthetic reporter gene construct, whereas activation of the cyclin D1 promoter by beta-catenin is refractory to p300 stimulation. Axis formation and activation of the beta-catenin target genes siamois and Xnr-3 in Xenopus embryos are sensitive to the E1A oncoprotein, a known inhibitor of p300/CBP. The C-terminus of beta-catenin interacts directly with a region overlapping the CH-3 domain of p300. p300 could participate in alleviating promoter repression imposed by chromatin structure and in recruiting the basal transcription machinery to promoters of particular Wnt target genes.

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@article{Hecht2000ThePA, title={The p300/CBP acetyltransferases function as transcriptional coactivators of beta-catenin in vertebrates.}, author={Andreas Hecht and Kris Vleminckx and Marc P Stemmler and Frans van Roy and Rolf Kemler}, journal={The EMBO journal}, year={2000}, volume={19 8}, pages={1839-50} }