The p23 Protein of Citrus Tristeza Virus Controls Asymmetrical RNA Accumulation

  title={The p23 Protein of Citrus Tristeza Virus Controls Asymmetrical RNA Accumulation},
  author={Tatineni Satyanarayana and Siddarame Gowda and Mar{\'i}a A. Ayll{\'o}n and M R Albiach-Mart{\'i} and Shailaja Rabindran and William O. Dawson},
  journal={Journal of Virology},
  pages={473 - 483}
ABSTRACT Citrus tristeza virus (CTV), a member of the Closteroviridae, has a 19.3-kb positive-stranded RNA genome that is organized into 12 open reading frames (ORFs) with the 10 3′ genes expressed via a nested set of nine or ten 3′-coterminal subgenomic mRNAs (sgRNAs). Relatively large amounts of negative-stranded RNAs complementary to both genomic and sgRNAs accumulate in infected cells. As is characteristic of RNA viruses, wild-type CTV produced more positive than negative strands, with the… 

Citrus tristeza virus p23: a unique protein mediating key virus–host interactions

The large RNA genome of Citrus tristeza virus (CTV; ca. 20 kb) contains 12 open reading frames, with the 3′-terminal one corresponding to a protein of 209 amino acids (p23) that is expressed from an

Effects of Modification of the Transcription Initiation Site Context on Citrus Tristeza Virus Subgenomic RNA Synthesis

Based on results, the CTV replication complex appears to initiate sgRNA synthesis with purines, preferably with adenylates, and is able to initiate synthesis using a nucleotide a few positions 5′ or 3′ of the native initiation nucleotide.

Molecular Virology and Pathogenicity of Citrus tristeza virus

Citrus tristeza virus (CTV) is the largest identified RNA virus infecting plants and the second largest worldwide after the animal Coronaviruses.

Citrus tristeza virus: Host RNA Silencing and Virus Counteraction.

The high accumulation of CTV sRNAs in two of the citrus hosts examined suggests that it is not their synthesis, but their function, the target of the RSS encoded by CTV: p25 (intercellular), p23 (intracellular) and p20 (both).

Protein–protein interactions between proteins of Citrus tristeza virus isolates

New information is provided on CTV protein interactions which will help for further understanding the biological functions and the domains responsible for CP and p20 self-interactions were mapped at the CP amino acids sites 41–84 and p 20 amino acids Sites 1–21 by Y2H.

Citrus tristeza virus co-opts glyceraldehyde 3-phosphate dehydrogenase for its infectious cycle by interacting with the viral-encoded protein p23

Key messageCitrus tristeza virus encodes a unique protein, p23, with multiple functional roles that include co-option of the cytoplasmic glyceraldehyde 3-phosphate dehydrogenase to facilitate the

The Complex Genetics of Citrus tristeza virus

The 2000 x 11 nm long bipolar flexuous filamentous particles of Citrus tristeza virus (CTV) (genus Closterovirus, family Closteroviridae) (Figure 1) contain a single-stranded positive-sense RNA



Kinetics of accumulation of citrus tristeza virus RNAs.

The kinetics of accumulation of genomic and sg RNAs and coat protein of the T36 isolate of CTV were similar in protoplasts of the natural host, citrus, and the experimental nonhost Nicotiana benthamiana, suggesting that its regulation was transcriptional.

Complete sequence of the citrus tristeza virus RNA genome.

Phylogenetic analysis of the three replication-associated domains, MT, HEL, and RdRp, indicates that CTV and BYV form a separate closterovirus lineage within the alpha-like supergroup of positive-strand RNA viruses.

Transcriptional strategy of closteroviruses: mapping the 5' termini of the citrus tristeza virus subgenomic RNAs

The data obtained suggest that the sgRNA transcription of CTV is dissimilar from the coronavirus transcription and consistent with the transcriptional mechanism of other Sindbis-like viruses, which might be successfully utilized by the closterovirus genome of up to 19.3 kb with multiple sgRNAs.

Characterization of the cis-acting elements controlling subgenomic mRNAs of citrus tristeza virus: production of positive- and negative-stranded 3'-terminal and positive-stranded 5'-terminal RNAs.

Citrus tristeza virus has an approximately 20-kb positive-sense RNA genome with two 5' ORFs translated from the genomic RNA and 10 3' genes expressed via nine or ten 3'-terminal subgenomic RNAs, which theoretically could produce 30-33 species of RNAs in infected cells.

Nucleotide sequence and organization of eight 3' open reading frames of the citrus tristeza closterovirus genome.

A specific four-gene module consisting of thehsp70 protein, the hsp90-related protein, a diverged copy of the CP, and the CP itself was found to be common in organization between CTV and beet yellows closterovirus, indicating that these viruses probably have evolved from a common ancestor.

Characterization of citrus tristeza virus subgenomic RNAs in infected tissue.

Citrus tristeza virus (CTV) specific RNAs extracted from infected citrus tissue were analyzed by Northern blot hybridization and found that the RNAs for the p20 and p23 ORFs were the most abundant and surpassed the amount of the p25 or capsid protein specific subgenomic RNA.

Role of the nonstructural polyproteins in alphavirus RNA synthesis.

This review will highlight research efforts directed toward understanding the synthesis of alphavirus RNA and its regulation and highlight the importance of knowing the number and rate of viral plus-strand RNA synthesis.

The 23-kDa protein coded by the 3'-terminal gene of citrus tristeza virus is an RNA-binding protein.

Gel retardation and UV crosslinking assays demonstrated that p23 has the ability to cooperatively bind single-stranded RNA in a non-sequence-specific manner, and the p23-RNA complex was stable at high salt concentrations, suggesting that interactions other than those between the negatively charged RNA and the basic region of p23 are involved.

Amplification of Citrus tristeza virus from a cDNA clone and infection of citrus trees.

Fulfilling Koch's postulates of the first pure culture of CTV in plants suggested that the major genotype of the CTV T36 population is the primary determinant of the symptom phenotype.