The oxidative TCA cycle operates during methanotrophic growth of the Type I methanotroph Methylomicrobium buryatense 5GB1.

@article{Fu2017TheOT,
  title={The oxidative TCA cycle operates during methanotrophic growth of the Type I methanotroph Methylomicrobium buryatense 5GB1.},
  author={Yanfen Fu and Yi Li and Mary E. Lidstrom},
  journal={Metabolic engineering},
  year={2017},
  volume={42},
  pages={
          43-51
        }
}

Oxygen-limited metabolism in the methanotroph Methylomicrobium buryatense 5GB1C

The results indicate that even under O2-starvation conditions, M. buryatense 5GB1 maintains a metabolic state representing a combination of fermentation and respiration metabolism, which is likely to be induced by low-O2 conditions.

Quantifying Methane and Methanol Metabolism of “Methylotuvimicrobium buryatense” 5GB1C under Substrate Limitation

A core consensus central metabolic flux phenotype is revealed across different growth conditions: a strong ribulose monophosphate cycle, a preference for the Embden-Meyerhof-Parnas pathway as the primary glycolytic pathway, and a tricarboxylic acid cycle showing small yet significant fluxes.

The Entner-Doudoroff Pathway Is an Essential Metabolic Route for Methylotuvimicrobium buryatense 5GB1C

The essentiality of the Entner-Doudoroff pathway is demonstrated for M. buryatense 5GB1C by creating a construct with edd-eda and gltA genes controlled by PBAD and PJ23101, respectively, which demonstrates that these genetic tools are applicable to this obligate methanotroph.

Metabolic flux analysis and metabolomics of Methylotrophs

It was demonstrated that the C3/C4 interconversion reactions, previously thought to be side reactions of little relevance to core metabolism, are critical to the ultimate values of growth rate and yield and provides targets for strain engineering towards either maximum growth rate, or maximum yield.

Core Metabolism Shifts during Growth on Methanol versus Methane in the Methanotroph Methylomicrobium buryatense 5GB1

The results suggest a systematic shift of active core metabolism in which increased flux occurred through both the Entner-Doudoroff pathway and the partial serine cycle, while the tricarboxylic acid cycle was incomplete, in contrast to growth on methane.

Genome-scale revealing the central metabolic network of the fast growing methanotroph Methylomonas sp. ZR1

Methylomonas sp. ZR1 was an isolated new methanotrophs that could utilize methane and methanol growing fast and synthesizing value added compounds such as lycopene. In this study, the genomic study

Physiology and Biochemistry of the Aerobic Methanotrophs

The availability of genome sequences of methanotrophs enables postgenomic studies to investigate the regulation of methane oxidation in the laboratory and in the environment by natural methanOTrophs and in laboratory or industrial conditions by platform organisms.

Role of the malic enzyme in metabolism of the halotolerant methanotroph Methylotuvimicrobium alcaliphilum 20Z

It is concluded that the malic enzyme is involved in the control of intracellular L-malate level in Mtm, the halotolerant obligate methanotroph Methylotuvimicrobium alcaliphilum 20Z.

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