The oxidative TCA cycle operates during methanotrophic growth of the Type I methanotroph Methylomicrobium buryatense 5GB1.

  title={The oxidative TCA cycle operates during methanotrophic growth of the Type I methanotroph Methylomicrobium buryatense 5GB1.},
  author={Yanfen Fu and Yi Li and Mary E. Lidstrom},
  journal={Metabolic engineering},

Oxygen-limited metabolism in the methanotroph Methylomicrobium buryatense 5GB1C

The results indicate that even under O2-starvation conditions, M. buryatense 5GB1 maintains a metabolic state representing a combination of fermentation and respiration metabolism, which is likely to be induced by low-O2 conditions.

Quantifying Methane and Methanol Metabolism of “Methylotuvimicrobium buryatense” 5GB1C under Substrate Limitation

A core consensus central metabolic flux phenotype is revealed across different growth conditions: a strong ribulose monophosphate cycle, a preference for the Embden-Meyerhof-Parnas pathway as the primary glycolytic pathway, and a tricarboxylic acid cycle showing small yet significant fluxes.

The Entner-Doudoroff Pathway Is an Essential Metabolic Route for Methylotuvimicrobium buryatense 5GB1C

The essentiality of the Entner-Doudoroff pathway is demonstrated for M. buryatense 5GB1C by creating a construct with edd-eda and gltA genes controlled by PBAD and PJ23101, respectively, which demonstrates that these genetic tools are applicable to this obligate methanotroph.

Metabolic flux analysis and metabolomics of Methylotrophs

It was demonstrated that the C3/C4 interconversion reactions, previously thought to be side reactions of little relevance to core metabolism, are critical to the ultimate values of growth rate and yield and provides targets for strain engineering towards either maximum growth rate, or maximum yield.

Core Metabolism Shifts during Growth on Methanol versus Methane in the Methanotroph Methylomicrobium buryatense 5GB1

The results suggest a systematic shift of active core metabolism in which increased flux occurred through both the Entner-Doudoroff pathway and the partial serine cycle, while the tricarboxylic acid cycle was incomplete, in contrast to growth on methane.

Genome-scale revealing the central metabolic network of the fast growing methanotroph Methylomonas sp. ZR1

Methylomonas sp. ZR1 was an isolated new methanotrophs that could utilize methane and methanol growing fast and synthesizing value added compounds such as lycopene. In this study, the genomic study

Physiology and Biochemistry of the Aerobic Methanotrophs

The availability of genome sequences of methanotrophs enables postgenomic studies to investigate the regulation of methane oxidation in the laboratory and in the environment by natural methanOTrophs and in laboratory or industrial conditions by platform organisms.

Role of the malic enzyme in metabolism of the halotolerant methanotroph Methylotuvimicrobium alcaliphilum 20Z

It is concluded that the malic enzyme is involved in the control of intracellular L-malate level in Mtm, the halotolerant obligate methanotroph Methylotuvimicrobium alcaliphilum 20Z.



Genome-scale metabolic reconstructions and theoretical investigation of methane conversion in Methylomicrobium buryatense strain 5G(B1)

The metabolic model can provide an effective tool for predicting metabolic parameters for different nutrients and genetic perturbations, and as such, should be valuable for metabolic engineering of the central metabolism of M. buryatense strains.

Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1

The results presented here represent the most comprehensive published bioreactor datasets for a gamma-proteobacterial methanotroph and show that metabolism by M. buryatense 5GB1 differs significantly for each of the four conditions tested.

Genetic Tools for the Industrially Promising Methanotroph Methylomicrobium buryatense

Genetic tools for Methylomicrobium buryatense, a haloalkaliphilic gammaproteobacterial (type I) methanotroph, are developed and validated, enabling metabolic engineering in this bacterium for industrial biocatalysis of methane.

13C-MFA delineates the photomixotrophic metabolism of Synechocystis sp. PCC 6803 under light- and carbon-sufficient conditions.

The current study employed (13) C isotopomers to delineate pathways in the cyanobacterium Synechocystis sp.

XoxF Acts as the Predominant Methanol Dehydrogenase in the Type I Methanotroph Methylomicrobium buryatense

Evidence is presented that XoxF acts as the predominant methanol dehydrogenase in a methanotroph, and it is demonstrated that micromolar amounts of lanthanides are sufficient to suppress MxaFI expression.

Electroporation-Based Genetic Manipulation in Type I Methanotrophs

This study developed electroporation techniques with a high transformation efficiency for three different type I methanotrophs, promoting the ability to perform fundamental research and industrial process development with these strains.

Taxonomic characterization of new alkaliphilic and alkalitolerant methanotrophs from soda lakes of the Southeastern Transbaikal region and description of Methylomicrobium buryatense sp.nov.

Comparative 16S rDNA sequencing showed that the newly isolated methanotrophs are related to membres of the Methylomicrobium genus, however, they differ from the known members of this genus by DNA-DNA relatedness.