The oxidation of arachidonic acid by the cyclooxygenase activity of purified prostaglandin H synthase: spin trapping of a carbon-centered free radical intermediate.

@article{Schreiber1986TheOO,
  title={The oxidation of arachidonic acid by the cyclooxygenase activity of purified prostaglandin H synthase: spin trapping of a carbon-centered free radical intermediate.},
  author={J{\"o}rg Schreiber and Thomas E. Eling and Ronald Paul Mason},
  journal={Archives of biochemistry and biophysics},
  year={1986},
  volume={249 1},
  pages={126-36}
}
The ESR spin trapping technique was used to study the first detectable radical intermediate in the oxidation of arachidonic acid by purified prostaglandin H synthase. The holoenzyme and the apoenzyme, reconstituted with either hematin or Mn2+ protoporphyrin IX, were investigated. Depending on the different types of enzyme activity present, arachidonic acid was oxidized to at least two free radicals. One of these radicals is thought to be the first ESR detectable radical intermediate in the… CONTINUE READING

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