The overexpressed hexahistidine-tagged human hexokinase type III is inhibited by D-glucose.

@article{Palma2002TheOH,
  title={The overexpressed hexahistidine-tagged human hexokinase type III is inhibited by D-glucose.},
  author={F. Dalla Palma and Deborah Agostini and Emanuela Polidori and Vilberto Stocchi},
  journal={Preparative biochemistry & biotechnology},
  year={2002},
  volume={32 4},
  pages={393-403}
}
Inhibition by its product, glucose, is a kinetic property of hexokinase type III. In this paper, we report the overexpression in Escherichia coli of human hexokinase type III. The recombinant enzyme was genetically fused with a hexahistidine peptide at the C-terminal end. This modification confers to the product the ability to bind the Ni2+ ion immobilised… CONTINUE READING