The outer membrane porin OmpW of Acinetobacter baumannii is involved in iron uptake and colistin binding.

Abstract

This study was undertaken to characterize functions of the outer membrane protein OmpW, which potentially contributes to the development of colistin- and imipenem-resistance in Acinetobacter baumannii. Reconstitution of OmpW in artificial lipid bilayers showed that it forms small channels (23 pS in 1 m KCl) and markedly interacts with iron and colistin, but not with imipenem. In vivo, (55) Fe uptake assays comparing the behaviours of ΔompW mutant and wild-type strains confirmed a role for OmpW in A. baumannii iron homeostasis. However, the loss of OmpW expression did not have an impact on A. baumannii susceptibilities to colistin or imipenem.

DOI: 10.1002/1873-3468.12050

Cite this paper

@article{CatelFerreira2016TheOM, title={The outer membrane porin OmpW of Acinetobacter baumannii is involved in iron uptake and colistin binding.}, author={Manuella Catel-Ferreira and Sara Mart{\'i} and Laurent Guillon and Luis M Jara and Ga{\"{e}l Coadou and Virginie Molle and Emeline Bouffartigues and Germ{\'a}n Bou and Isabelle Shalk and Thierry Jouenne and Xavier Vila-Farr{\'e}s and Emmanuelle D{\'e}}, journal={FEBS letters}, year={2016}, volume={590 2}, pages={224-31} }