The origins and evolution of freeze-etch electron microscopy.

  title={The origins and evolution of freeze-etch electron microscopy.},
  author={John E. Heuser},
  journal={Journal of electron microscopy},
  volume={60 Suppl 1},
The introduction of the Balzers freeze-fracture machine by Moor in 1961 had a much greater impact on the advancement of electron microscopy than he could have imagined. Devised originally to circumvent the dangers of classical thin-section techniques, as well as to provide unique en face views of cell membranes, freeze-fracturing proved to be crucial for developing modern concepts of how biological membranes are organized and proved that membranes are bilayers of lipids within which proteins… CONTINUE READING