The origin of transfer (oriT) of the conjugative plasmid R46: Characterization by deletion analysis and DNA sequencing


The origin of transfer (oriT) is the sequence within which conjugal transfer of plasmid DNA is initiated, and is absolutely required in cis for plasmid mobilization. We have cloned oriT from the 52 kb IncN plasmid R46 on a 600 bp fragment, and mapped the limits of the relevant sequence by deletion analysis and transposon mutagenesis. The nucleotide sequence… (More)
DOI: 10.1007/BF00330445


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