The origin of enantioselectivity in aldolase antibodies: crystal structure, site-directed mutagenesis, and computational analysis.

Abstract

Catalytic aldolase antibodies, generated by reactive immunization, catalyze the aldol reaction with the efficiency of natural enzymes, but accept a much broader range of substrates. Two separate groups of aldolase antibodies that catalyze the same aldol reactions with antipodal selectivity were analyzed by comparing their amino acid sequences with their… (More)

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@article{Zhu2004TheOO, title={The origin of enantioselectivity in aldolase antibodies: crystal structure, site-directed mutagenesis, and computational analysis.}, author={Xueyong Zhu and Fujie Tanaka and Yunfeng Hu and Andreas von Heine and Roberta P. Fuller and Guofu Zhong and Arthur J. Olson and Richard Allen Lerner and Carlos F. Barbas and Ian A. Wilson}, journal={Journal of molecular biology}, year={2004}, volume={343 5}, pages={1269-80} }