The organization of the core proteins of the yeast spindle pole body.

  title={The organization of the core proteins of the yeast spindle pole body.},
  author={Eric G. D. Muller and Brian E. Snydsman and Isabella Novik and Dale Warren Hailey and Daniel R. Gestaut and Christine A Niemann and Eileen O'Toole and Tom H Giddings and Bryan A Sundin and Trisha N. Davis},
  journal={Molecular biology of the cell},
  volume={16 7},
The spindle pole body (SPB) is the microtubule organizing center of Saccharomyces cerevisiae. Its core includes the proteins Spc42, Spc110 (kendrin/pericentrin ortholog), calmodulin (Cmd1), Spc29, and Cnm67. Each was tagged with CFP and YFP and their proximity to each other was determined by fluorescence resonance energy transfer (FRET). FRET was measured by a new metric that accurately reflected the relative extent of energy transfer. The FRET values established the topology of the core… CONTINUE READING
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