The organization of ribosomal RNA processing correlates with the distribution of nucleolar snRNAs.

@article{Beven1996TheOO,
  title={The organization of ribosomal RNA processing correlates with the distribution of nucleolar snRNAs.},
  author={Alison F. Beven and Rebecca Lee and Moe Razaz and David J. Leader and John W. S. Brown and Peter J. Shaw},
  journal={Journal of cell science},
  year={1996},
  volume={109 ( Pt 6)},
  pages={
          1241-51
        }
}
We have analyzed the organization of pre-rRNA processing by confocal microscopy in pea root cell nucleoli using a variety of probes for fluorescence in situ hybridization and immunofluorescence. Our results show that transcript processing within the nucleolus is spatially highly organized. Probes to the 5' external transcribed spacer (ETS) and first internal transcribed spacer (ITS1) showed that the excision of the ETS occurred in a sub-region of the dense fibrillar component (DFC), whereas the… CONTINUE READING

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