The organization and complete nucleotide sequence of the PstI restriction-modification system.

@article{Walder1984TheOA,
  title={The organization and complete nucleotide sequence of the PstI restriction-modification system.},
  author={Roxanne Y. Walder and Joseph A Walder and J. E. Donelson},
  journal={The Journal of biological chemistry},
  year={1984},
  volume={259 12},
  pages={8015-26}
}
We have determined the nucleotide sequence of a 4.0-kilobase DNA fragment containing the genes of the PstI restriction-modification system. Two large open reading frames were identified within the sequence and were ascribed to the restriction enzyme and methylase by the analysis of a series of deletion mutants. The two genes are encoded on opposite DNA strands, and hence must be transcribed from separate promoters rather than as a polycistronic message. The sequence of the first 10 amino acids… CONTINUE READING