The order of expression is a key factor in the production of active transglutaminase in Escherichia coli by co-expression with its pro-peptide

@inproceedings{Liu2011TheOO,
  title={The order of expression is a key factor in the production of active transglutaminase in Escherichia coli by co-expression with its pro-peptide},
  author={Song Liu and Dongxu Zhang and Miao Wang and Wenjing Cui and Kangkang Chen and Guocheng C. Du and Jian Chen and Zhemin Zhou},
  booktitle={Microbial cell factories},
  year={2011}
}
BACKGROUND Streptomyces transglutaminase (TGase) is naturally synthesized as zymogen (pro-TGase), which is then processed to produce active enzyme by the removal of its N-terminal pro-peptide. This pro-peptide is found to be essential for overexpression of soluble TGase in E. coli. However, expression of pro-TGase by E. coli requires protease-mediated activation in vitro. In this study, we developed a novel co- expression method for the direct production of active TGase in E. coli. RESULTS A… CONTINUE READING
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