The optics and performance of dual-focus fluorescence correlation spectroscopy.

  title={The optics and performance of dual-focus fluorescence correlation spectroscopy.},
  author={Thomas Dertinger and Anastasia Loman and Benjamin Ewers and Claus B M{\"u}ller and Benedikt Kraemer and J{\"o}rg Enderlein},
  journal={Optics express},
  volume={16 19},
Fluorescence correlation spectroscopy (FCS) is an important spectroscopic technique which can be used for measuring the diffusion and thus size of fluorescing molecules at pico- to nanomolar concentrations. Recently, we introduced an extension of conventional FCS, which is called dual-focus FCS (2fFCS) and allows absolute diffusion measurements with high precision and repeatability. It was shown experimentally that the method is robust against most optical and sample artefacts which are… CONTINUE READING


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