The open reading frame of bamboo mosaic potexvirus satellite RNA is not essential for its replication and can be replaced with a bacterial gene.

@article{Lin1996TheOR,
  title={The open reading frame of bamboo mosaic potexvirus satellite RNA is not essential for its replication and can be replaced with a bacterial gene.},
  author={N. S. Lin and Yeon Su Lee and Biing Yuan Lin and Chi Wai Lee and Yung Hisang Hsu},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1996},
  volume={93 7},
  pages={3138-42}
}
A satellite RNA of 836 nt depends on the bamboo mosaic potexvirus (BaMV) for its replication and encapsulation. The BaMV satellite RNA (satBaMV) contains a single open reading frame encoding a 20-kDa nonstructural protein. A full-length infectious cDNA clone has been generated downstream of the T7 RNA polymerase promoter. To investigate the role of the 20-kDa protein encoded by satBaMV, satBaMV transcripts containing mutations in the open reading frame were tested for their ability to replicate… CONTINUE READING
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