The number of large ribosomal RNA genes in Leptospira interrogans and Leptospira biflexa.

@article{Fukunaga1989TheNO,
  title={The number of large ribosomal RNA genes in Leptospira interrogans and Leptospira biflexa.},
  author={Masahito Fukunaga and Ichiji Mifuchi},
  journal={Microbiology and immunology},
  year={1989},
  volume={33 6},
  pages={459-66}
}
We determined the number of large ribosomal RNA genes in five strains of Leptospira by hybridization of 15 restriction endonuclease digests of genomic DNA to the [32P]-labeled fragment of 23s rRNA gene. Almost all the restriction gels gave two radioactive bands. The conclusion from these results is that there are at least two rRNA genes in these leptospiral strains. Furthermore, the hybridization patterns of L. icterohaemorrhagiae strains Ictero No. I and RGA are almost identical. The number of… CONTINUE READING

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