The nucleotides on the stem-loop RNA structure in the junction region of the hepatitis E virus genome are critical for virus replication.

@article{Cao2010TheNO,
  title={The nucleotides on the stem-loop RNA structure in the junction region of the hepatitis E virus genome are critical for virus replication.},
  author={Dianjun Cao and Y. Wayne Huang and X B Meng},
  journal={Journal of virology},
  year={2010},
  volume={84 24},
  pages={13040-4}
}
The roles of conserved nucleotides on the stem-loop (SL) structure in the intergenic region of the hepatitis E virus (HEV) genome in virus replication were determined by using Huh7 cells transfected with HEV SL mutant replicons containing reporter genes. One or two nucleotide mutations of the AGA motif on the loop significantly reduced HEV replication, and three or more nucleotide mutations on the loop abolished HEV replication. Mutations on the stem and of the subgenome start sequence also… CONTINUE READING