The nucleotide sequence of the tyrosinase gene from Streptomyces antibioticus and characterization of the gene product.

@article{Bernan1985TheNS,
  title={The nucleotide sequence of the tyrosinase gene from Streptomyces antibioticus and characterization of the gene product.},
  author={Valerie S. Bernan and David Filpula and Wayne K. Herber and Mervyn Bibb and E L Katz},
  journal={Gene},
  year={1985},
  volume={37 1-3},
  pages={101-10}
}
The sequence of a 1.56-kb DNA fragment containing the tyrosinase gene (mel) from Streptomyces antibioticus was determined and the Mr (30612) and amino acid (aa) sequence of the protein were deduced from the nucleotide (nt) sequence. Intracellular and extracellular tyrosinase from S. antibioticus, transformed with pIJ702 (containing mel), were purified to homogeneity; the Mr (29 500), as determined by Sephadex G-75 chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS… CONTINUE READING