The nucleoporin Nup60p functions as a Gsp1p–GTP-sensitive tether for Nup2p at the nuclear pore complex

@article{Denning2001TheNN,
  title={The nucleoporin Nup60p functions as a Gsp1p–GTP-sensitive tether for Nup2p at the nuclear pore complex},
  author={Daniel Denning and Brook Mykytka and Nadia P. C. Allen and Lan Huang and Al Burlingame and Michael Rexach},
  journal={The Journal of Cell Biology},
  year={2001},
  volume={154},
  pages={937 - 950}
}
The nucleoporins Nup60p, Nup2p, and Nup1p form part of the nuclear basket structure of the Saccharomyces cerevisiae nuclear pore complex (NPC). Here, we show that these necleoporins can be isolated from yeast extracts by affinity chromatography on karyopherin Kap95p-coated beads. To characterize Nup60p further, Nup60p-coated beads were used to capture its interacting proteins from extracts. We find that Nup60p binds to Nup2p and serves as a docking site for Kap95p-Kap60p heterodimers and… CONTINUE READING
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