The nucleoid binding protein H-NS acts as an anti-channeling factor to favor intermolecular Tn10 transposition and dissemination.

@article{Singh2008TheNB,
  title={The nucleoid binding protein H-NS acts as an anti-channeling factor to favor intermolecular Tn10 transposition and dissemination.},
  author={Randeep Kaur Singh and Janine Liburd and Simon J. Wardle and David B. Haniford},
  journal={Journal of molecular biology},
  year={2008},
  volume={376 4},
  pages={950-62}
}
Dissemination of the bacterial transposon Tn10 is limited by target site channeling, a process wherein the transposon ends are forced to interact with and insert into a target site located within the transposon. Integration host factor (IHF) promotes this self-destructive event by binding to the transpososome and forming a DNA loop close to one or both transposon ends; this loop imposes geometric and topological constraints that are responsible for channeling. We demonstrate that a second 'host… CONTINUE READING