The nuclease activity of the yeast DNA2 protein, which is related to the RecB-like nucleases, is essential in vivo.

@article{Budd2000TheNA,
  title={The nuclease activity of the yeast DNA2 protein, which is related to the RecB-like nucleases, is essential in vivo.},
  author={Martin E. Budd and Wonhie Choe and Judith L. Campbell},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 22},
  pages={16518-29}
}
Saccharomyces cerevisiae Dna2 protein is required for DNA replication and repair and is associated with multiple biochemical activities: DNA-dependent ATPase, DNA helicase, and DNA nuclease. To investigate which of these activities is important for the cellular functions of Dna2, we have identified separation of function mutations that selectively inactivate the helicase or nuclease. We describe the effect of six such mutations on ATPase, helicase, and nuclease after purification of the mutant… CONTINUE READING

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An Overview of the Molecular Mechanisms of Recombinational DNA Repair.

Cold Spring Harbor perspectives in biology • 2015
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References

Publications referenced by this paper.
Showing 1-2 of 2 references

Genetic Recombination (Kucherlapaatic

A. F. Taylor
American Society for Microbiology, • 1988

Cold Spring Harbor Symp

T. Kogoma
Quant . Biol . • 1968

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