The novel VIP-like hypothalamic polypeptide PACAP interacts with high affinity receptors in the human neuroblastoma cell line NB-OK.

@article{Cauvin1990TheNV,
  title={The novel VIP-like hypothalamic polypeptide PACAP interacts with high affinity receptors in the human neuroblastoma cell line NB-OK.},
  author={Aline Cauvin and Louis Buscail and P Gourlet and Philippe De Neef and D Gossen and Akira Arimura and Atsuro Miyata and D. H. Coy. E. J. Coy and Patrick Robberecht and Jean Christophe},
  journal={Peptides},
  year={1990},
  volume={11 4},
  pages={773-7}
}
We investigated the ability of two forms of Pituitary Adenylate Cyclase Activating Polypeptide [PACAP-38, the 38 amino acid peptide isolated from ovine hypothalamus, and PACAP-27, a shorter N-terminal (1-27) amidated version] to interact with specific receptors in membranes from the human neuroblastoma cell line NB-OK. [125I]PACAP-27 bound rapidly and specifically to one class of high affinity sites (Kd 0.5 nM). VIP inhibited [125I]PACAP-27 binding 300- to 1000-fold less potently than PACAP-27… CONTINUE READING