The non-specific membrane binding properties of delta9-tetrahydrocannabinol and the effects of various solubilizers.

@article{Roth1979TheNM,
  title={The non-specific membrane binding properties of delta9-tetrahydrocannabinol and the effects of various solubilizers.},
  author={Sheldon H. Roth and Patrick J. Williams},
  journal={The Journal of pharmacy and pharmacology},
  year={1979},
  volume={31 4},
  pages={224-30}
}
The binding of [3H]delta9-tetrahydrocannabinol to crude and purified synaptosomal membrane suspended in either Krebs solution or 10 mM sodium phosphate buffer was examined. The membrane/buffer partition coefficient was found to be 12,500, and was constant over a free concentration range of 10(-8) to 10(-6)M. Binding was similar in both suspending media, and to both crude and purified synaptosomal membrane. The solubilizing agent Cremophor E.L. (8 microgram ml-1) decreased the partition… CONTINUE READING