The new frontier of genome engineering with CRISPR-Cas9

@article{Doudna2014TheNF,
  title={The new frontier of genome engineering with CRISPR-Cas9},
  author={J. Doudna and E. Charpentier},
  journal={Science},
  year={2014},
  volume={346}
}
Background Technologies for making and manipulating DNA have enabled advances in biology ever since the discovery of the DNA double helix. But introducing site-specific modifications in the genomes of cells and organisms remained elusive. Early approaches relied on the principle of site-specific recognition of DNA sequences by oligonucleotides, small molecules, or self-splicing introns. More recently, the site-directed zinc finger nucleases (ZFNs) and TAL effector nucleases (TALENs) using the… Expand
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References

SHOWING 1-10 OF 159 REFERENCES
RNA-guided genome editing in plants using a CRISPR-Cas system.
RNA-programmed genome editing in human cells
Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation
...
1
2
3
4
5
...