The neutral comet assay detects double strand DNA damage in selected and unselected human spermatozoa of normospermic donors.

@article{Kooij2004TheNC,
  title={The neutral comet assay detects double strand DNA damage in selected and unselected human spermatozoa of normospermic donors.},
  author={Rolph van Kooij and Peter de Boer and Johanna M. T. de Vreeden-Elbertse and N A Ganga and Nita Singh and Egbert R. te Velde},
  journal={International journal of andrology},
  year={2004},
  volume={27 3},
  pages={140-6}
}
The occurrence of DNA breaks in human sperm is of concern to genetic safety in artificial reproduction techniques. Here, we have explored the neutral comet assay (NCA) for evaluating the frequency of spermatozoa with double strand (ds) DNA breaks in normospermic donors. The NCA results into DNA tail formation by fibre extension and by the separation of DNA fragments. Gamma-irradiated native, lysed and lysed plus RNA and protein degraded human sperm nuclei have been used to assess sensitivity… CONTINUE READING
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