The neuronal growth-associated protein GAP-43 induces filopodia in non-neuronal cells.

@article{Zuber1989TheNG,
  title={The neuronal growth-associated protein GAP-43 induces filopodia in non-neuronal cells.},
  author={Mauricio X. Zuber and D W Goodman and Larry R. Karns and Mark C Fishman},
  journal={Science},
  year={1989},
  volume={244 4909},
  pages={
          1193-5
        }
}
The neuron-specific protein GAP-43 is associated with the membrane of the nerve growth cone and thus may be important to the activity of this distinctive neuronal structure. Transient transfection of COS and NIH 3T3 cells with appropriate vectors resulted in expression of GAP-43 in these non-neuronal cells; as in neurons, transfected GAP-43 associated with the membrane. In addition, many long fine filopodial processes extended from the periphery of such transfected cells. Stable CHO cell lines… Expand
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References

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A protein induced during nerve growth (GAP-43) is a major component of growth-cone membranes
TLDR
Immunohistochemical localization of GAP-43 in frozen sections of developing brain indicates that the protein is specifically associated with neuropil areas containing growth cones and immature synaptic terminals, and supports the proposal that Gap-43 plays a role in axon growth. Expand
Growth-associated protein, GAP-43, a polypeptide that is induced when neurons extend axons, is a component of growth cones and corresponds to pp46, a major polypeptide of a subcellular fraction enriched in growth cones.
TLDR
It is shown by means of subcellular fractionation and immunohistochemical localization that GAP-43 is a component of neuronal growth cones as well as growing neurites; it is similar to a major phosphoprotein, pp46, of a growth cone-enriched sub cellular fraction. Expand
Cloning of complementary DNA for GAP-43, a neuronal growth-related protein.
GAP-43 is one of a small subset of cellular proteins selectively transported by a neuron to its terminals. Its enrichment in growth cones and its increased levels in developing or regeneratingExpand
Dual regulation of GAP-43 gene expression by nerve growth factor and glucocorticoids.
TLDR
The regulation of GAP-43 is consistent with the notion that this gene is bimodally regulated during these cell fate decisions, with nuclear run-on experiments showing that the steroid repression is mediated at the level of gene transcription but that the NGF effect is likely to be posttranscriptional. Expand
Neuronal cytomechanics: the actin-based motility of growth cones.
TLDR
New results from electronically enhanced light microscopy of living growth cones are helping to show how actin-based forces guide neurite growth and synapse formation. Expand
Growth-associated protein GAP-43 is expressed selectively in associative regions of the adult human brain.
TLDR
Results suggest that a restricted subset of cortical and hippocampal neurons may be specialized for synaptic remodeling and might play a role in information storage in the human brain. Expand
Axonally transported proteins associated with axon growth in rabbit central and peripheral nervous systems
TLDR
Comparisons of rapidly transported proteins in growing and nongrowing axons in rabbits suggest that the neuronal growth state can be defined as an altered program of gene expression exemplified in part by the expression of GAP genes whose products are involved in critical growth-specific functions. Expand
A membrane phosphoprotein associated with neural development, axonal regeneration, phospholipid metabolism, and synaptic plasticity
TLDR
It is shown that a membrane phosphoprotein initially studied independently by several laboratories and given different designations, is in fact the same protein, which may play a general role in the formation of synaptic relationships during development or regeneration, and a continuing role inThe functional modulation of certain synapses throughout life. Expand
Cloning of human GAP 43: Growth association and ischemic resurgence
TLDR
Analysis of postmortem human brain tissue disclosed uniformly high expression of GAP-43 throughout the neonatal brain, whereas in the adult brain high levels of G AP-43 persist only in discrete regions, suggesting a role for Gap-43 in remodeling and repair of mature CNS neurons. Expand
Changes in axonally transported proteins during axon regeneration in toad retinal ganglion cells
TLDR
The modulation of these proteins after axotomy is consistent with the possibility that they are involve in growth-specific functions and that the altered expression of a small number of genes is a crucial regulatory event in the transition of a mature neuron to a growth state. Expand
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