The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.

@article{Buckanovich1997TheNR,
  title={The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.},
  author={Ronald J Buckanovich and Robert B Darnell},
  journal={Molecular and cellular biology},
  year={1997},
  volume={17 6},
  pages={3194-201}
}
Nova-1, an autoantigen in paraneoplastic opsoclonus myoclonus ataxia (POMA), a disorder associated with breast cancer and motor dysfunction, is a neuron-specific nuclear RNA binding protein. We have identified in vivo Nova-1 RNA ligands by combining affinity-elution-based RNA selection with protein-RNA immunoprecipitation. Starting with a pool of approximately 10(15) random 52-mer RNAs, we identified long stem-loop RNA ligands that bind to Nova-1 with high affinity (Kd of approximately 2 nM… CONTINUE READING

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Stereospecific recognition of tryptophan agarose by in vitro selected RNA

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