The need for transparency and good practices in the qPCR literature

  title={The need for transparency and good practices in the qPCR literature},
  author={Stephen Bustin and Vladimir Benes and Jeremy Garson and Jan Hellemans and Jim F Huggett and Mikael Kubista and Reinhold Mueller and Tania Nolan and Michael W. Pfaffl and Gregory L. Shipley and Carl T. Wittwer and Peter Schjerling and Philip J. R. Day and M{\'o}nica Abreu and Bego{\~n}a Aguado and Jean-François Beaulieu and Anneleen Beckers and Sara Bogaert and John A. Browne and Fernando Carrasco-Ramiro and Liesbeth Ceelen and Kate L. Ciborowski and Pieter Cornillie and St{\'e}phanie Coulon and Ann Cuypers and Sara De Brouwer and Leentje De Ceuninck and Jurgen De Craene and H{\'e}l{\`e}ne De Naeyer and Ward De Spiegelaere and Kato Deckers and Annelies Dheedene and Kaat Durinck and Margarida Ferreira-Teixeira and Annelies Fieuw and Jack M Gallup and Sandra Gonzalo-Flores and Karen Goossens and Femke Heindryckx and Elizabeth Herring and Hans Hoenicka and Laura Icardi and Rolf Jaggi and Farzad Javad and Michael Karampelias and Frederick S B Kibenge and Molly J. T. Kibenge and Candy Kumps and Irina Lambertz and Tim Lammens and Amelia Markey and Peter Messiaen and Evelien Mets and Sofia Morais and Alberto Mudarra-Rubio and Justine K. Nakiwala and Hilde Nelis and P{\aa}l A Olsvik and Claudina Angela P{\'e}rez-Novo and Michelle Plusquin and Tony Remans and Ali Rihani and Paulo Rodrigues-Santos and Pieter Rondou and Rebecca Sanders and Katharina Schmidt-Bleek and Kerstin Skovgaard and Karen H. G. Smeets and Laura Tabera and Stefan Toegel and Tim Acker and Wim Van den Broeck and J. C. van der Meulen and Mireille Van Gele and Gert Van Peer and Mario Van Poucke and Nadine van Roy and Sarah Vergult and Joris Wauman and Marina Tshuikina-Wiklander and Erik Willems and Sara Zaccara and Fjoralba Zeka and Jo Vandesompele},
  journal={Nature Methods},
Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not. 
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