The nature of the interaction between thyroid stimulating gamma G-globulin (long-acting thyroid stimulator) and thyroid tissue.

  title={The nature of the interaction between thyroid stimulating gamma G-globulin (long-acting thyroid stimulator) and thyroid tissue.},
  author={B. Rees Smith and Donald S. Munro},
  journal={Biochimica et biophysica acta},
  volume={208 2},
  • B. SmithD. Munro
  • Published 12 May 1970
  • Biology, Medicine
  • Biochimica et biophysica acta
18 Citations

The production of thyroid-stimulating immunoglobulin (TSI) by lymphocytes from patients with Graves’ disease cultured with human thyroid subcellular fractions

The results suggest that the normal human thyroid plasma membrane component may be the most important subcellular fraction acting as an antigenic stimulant of lymphocytes from patients with Graves’ disease (causing them to produce thyroid-stimulating immunoglobulin).


Diamide and Dithiothreitol indicates that TSH receptors and the thyroidal factor capable of neutralizing LATS share a common property in terms of the effects of SH reagents and is compatible with the idea that the LATS neutralizing activity of thyroidal crude membrane fraction is due to binding by the TSH receptor or its fragments.

The lipolytic activity of long-acting thyroid stimulator.

Binding of thyrotrophin to low molecular weight fragments of human thyroid membranes.

The LATS absorbing activity from thyroid homogenates which is associated with the soluble thyroid fraction (4S-LAA) binds TSH in addition to LATS. This may indicate that 4S-LAA originates from the

TSH receptor antibodies.

Now the M22-TSHR structure is known at the atomic level, rational design of specific low-molecular-weight inhibitors of the TSHR- TSHR autoantibody interaction is feasible and should provide key insights as to the differences between TSHRs with blocking and with stimulating activities.

Incidence and specificities of labeled thyrotropic hormone (TSH) binding immunoglobulins (LTB-Igs) in patients with Graves' disease and other thyroid disorders.

Using [125I]bTSH, labeled TSH binding (LTB) in sera from 203 patients with various thyroid disorders was studied, revealing that apparently higher incidence of increased LTB in untreated Graves' patients than the others, though some increased L TB cases were also observed in patients with inactive Graves' or other thyroid disorders.

Kinetics of thyrotropin-stimulating hormone (TSH) and thyroid-stimulating antibody binding and action on the TSH receptor in intact TSH receptor-expressing CHO cells.

Analysis of the data suggest that serum TSAb are oligoclonal antibodies sets, at low concentrations, with a high affinity for the TSH receptor; ionic interactions are involved in the action of TSAb on the Tsh receptor; and TSAb activation of the T SH receptor is at least a two-step process.



Inhibition of long-acting thyroid stimulator by thyroid particulate fractions.

In an effort to demonstrate such a reaction, serum containing LATS was incubated with insoluble fractions of thyroid tissue and evaluated the effects of this incubation by measuring LATS in the serum freed of suspended material by centrifugation.

The long‐acting thyroid stimulator

It has been suggested that LATS is an autoantibody to a thyroid component, and the level correlates with several parameters of thyroid activity.

On the immunological nature of the long acting thyroid stimulator.

  • G. BeallD. Solomon
  • Biology, Medicine
    The Journal of clinical endocrinology and metabolism
  • 1966
It was not possible to transfer LATS activity to new immunoglobulin G (IgG) molecules in a dialysis bath or bound to cellulose, thus supporting the idea that LATS is an integral part of the IgG molecule.

Studies on the antigen reacting with the thyroid-stimulating immunoglobulin (LATS) in thyrotoxicosis.

The results obtained are consistent with the hypothesis that LATS is an auto-antibody reacting with the thyroid; the possibility that the antigen is a constituent of the plasma membrane of the epithelial cells is discussed.

The proteolysis of immunoglobulin G with long-acting thyroid-stimulating activity.

1. Proteolysis of immunoglobulin G with long-acting thyroid-stimulating activity with papain and pepsin shortened the duration of thyroid stimulation in mice prepared for the McKenzie (1958)

The bioassay of thyrotropin in serum.

Endogenous thyrotropic activity was shown to travel on electrophoresis with gamma globulin of such sera and was also present only in the Gamma globulin fraction (II) of Cohn fractions of human plasma.

Extraction of thyrotrophin from pituitary glands, mouse pituitary tumors, and blood plasma by percolation.

A percolation method of extraction of thyrotrophin from dry powders of anterior pituitary glands, mousepituitary tumors and blood plasma has been developed and yields were estimated to be over 50% of the potency in about 2% of of the weight.

The gel-filtration behaviour of proteins related to their molecular weights over a wide range.

1. Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on