The mutation F227I increases the coupling of metal ion transport in DCT1.

@article{Nevo2004TheMF,
  title={The mutation F227I increases the coupling of metal ion transport in DCT1.},
  author={Yaniv Nevo and Nathan Alfred Nelson},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 51},
  pages={53056-61}
}
Metal ion transport by DCT1, a member of the natural resistance-associated macrophage protein family, is driven by protons. The stoichiometry of the proton to metal ion is variable, and under optimal transport conditions, more than 10 protons are co-transported with a single metal ion. To understand this phenomenon better, we used site-directed mutagenesis of DCT1 and analyzed the mutants by complementation of yeast suppressor of mitochondria import function-null mutants and electrophysiology… CONTINUE READING