The mouthwash: a non-invasive sampling method to study cytokine gene polymorphisms.

@article{Laine2000TheMA,
  title={The mouthwash: a non-invasive sampling method to study cytokine gene polymorphisms.},
  author={Marja L Laine and Maria A. Farré and J. Bart A. Crusius and Arie Jan van Winkelhoff and A. S. Pe{\~n}a},
  journal={Journal of periodontology},
  year={2000},
  volume={71 8},
  pages={
          1315-8
        }
}
BACKGROUND We describe a simple, non-invasive mouthwash sampling method for rapid DNA isolation to detect cytokine gene polymorphisms. In the present paper, interleukin- 1beta(IL-1B) and interleukin-1 receptor antagonist (IL-1RN) gene polymorphisms were studied. METHODS Two mouthwash samples and blood samples were collected from 11 healthy individuals. The second mouthwash sample was stored for 7 days at room temperature. Polymerase chain reaction amplification was used to identify a bi… 
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