The molecular structure of microtubule-associated protein 1A (MAP1A) in vivo and in vitro. An immunoelectron microscopy and quick-freeze, deep-etch study.

@article{Shiomura1987TheMS,
  title={The molecular structure of microtubule-associated protein 1A (MAP1A) in vivo and in vitro. An immunoelectron microscopy and quick-freeze, deep-etch study.},
  author={Y. Shiomura and Nobutaka Hirokawa},
  journal={The Journal of neuroscience : the official journal of the Society for Neuroscience},
  year={1987},
  volume={7 5},
  pages={1461-9}
}
We studied the distribution of microtubule-associated protein 1A (MAP1A) in Purkinje cell dendrites by means of electronmicroscopic immunocytochemistry, using a monoclonal antibody (McAb) against MAP1A; this was combined with the observation of the 3-dimensional cytoskeletal ultrastructure in dendrites via the quick-freeze, deep-etch technique (QF-DE). We prepared a McAb against rat brain MAP1. This McAb recognized MAP1A on a nitrocellulose filter through use of the immunoblotting method, and… CONTINUE READING
27 Citations
36 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 27 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 36 references

Cytoskeletal architecture of reactivated crayfish axons, with special reference to crossbridges among microtubules and between microtubules and membrane organelles

  • N. Hirokawa, H. Yorifuji
  • Cell Motil
  • 1986
1 Excerpt

A monoclonal antibody to tau-factor localizes predominantly in axons

  • L. I. Binder, A. Frankfurter, L. I. Rebhun
  • J. Cell Biol. (Abstr.)
  • 1984
2 Excerpts

Similar Papers

Loading similar papers…