The molecular organization of human alpha 2-macroglobulin. An immunoelectron microscopic study with monoclonal antibodies.

@article{Delain1988TheMO,
  title={The molecular organization of human alpha 2-macroglobulin. An immunoelectron microscopic study with monoclonal antibodies.},
  author={Etienne Delain and M Barray and Jacqueline Tapon-Bretaudi{\`e}re and F Pochon and Peter Marynen and Jean Jacques Cassiman and Herman Van den Berghe and Fred van Leuven},
  journal={The Journal of biological chemistry},
  year={1988},
  volume={263 6},
  pages={2981-9}
}
To study the three-dimensional organization of alpha 2-macroglobulin (alpha 2M) from human plasma, immunoelectron microscopy of negatively stained specimens was used. A panel of monoclonal antibodies (mAb) with specificities typical for the two major conformers of alpha 2M (native and protease-transformed) was explored. The mAb have been selected and were classified biochemically as specific for either native or transformed alpha 2M or as reactive with both conformers. Furthermore, among the… CONTINUE READING

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