The molecular mechanisms underlying BiP-mediated gating of the Sec61 translocon of the endoplasmic reticulum

@article{Alder2005TheMM,
  title={The molecular mechanisms underlying BiP-mediated gating of the Sec61 translocon of the endoplasmic reticulum},
  author={Nathan N. Alder and Ying W. Shen and Jeffrey L Brodsky and Linda M Hendershot and Arthur E. Johnson},
  journal={The Journal of Cell Biology},
  year={2005},
  volume={168},
  pages={389 - 399}
}
The Sec61 translocon of the endoplasmic reticulum membrane forms an aqueous pore that is gated by the lumenal Hsp70 chaperone BiP. We have explored the molecular mechanisms governing BiP-mediated gating activity, including the coupling between gating and the BiP ATPase cycle, and the involvement of the substrate-binding and J domain-binding regions of BiP. Translocon gating was assayed by measuring the collisional quenching of fluorescent probes incorporated into nascent chains of translocation… CONTINUE READING

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