The molecular biology of histochemical staining by cationic phthalocyanin dyes: the design of replacements for Alcian Blue.

@article{Scott1980TheMB,
  title={The molecular biology of histochemical staining by cationic phthalocyanin dyes: the design of replacements for Alcian Blue.},
  author={J. E. Scott},
  journal={Journal of microscopy},
  year={1980},
  volume={119 3},
  pages={373-81}
}
Four cationic copper phthalocyanin dyes are compared with Alcian Blue 8G for specificity of interaction with a range of natural and synthetic polyanions. The CEC system is used to estimate dye-substrate affinities. The results support the hypothesis that the tissue staining pattern of Alcian Blue is determined by (a) exclusion of the dye from polynucleotide stacked base pairs by steric hindrance due to bulky substituents on the chromophore and (b) the presence of pendant quaternary charges… CONTINUE READING

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