The mitochondrial permeability transition initiates autophagy in rat hepatocytes

@article{Elmore2001TheMP,
  title={The mitochondrial permeability transition initiates autophagy in rat hepatocytes},
  author={Steven P. Elmore and Ting Qian and Sherry F. Grissom and John J. Lemasters},
  journal={The FASEB Journal},
  year={2001},
  volume={15}
}
Cells degrade excess and effete organelles by the process of autophagy. Autophagic stimulation of rat hepatocytes by serum deprivation and glucagon (1 µM) caused a fivefold increase of spontaneously depolarizing mitochondria to about 1.5% of total mitochondria after 90 min. Cyclosporin A (CsA, 5 µM), an immunosuppressant that blocks the mitochondrial permeability transition (MPT), prevented this depolarization. Depolarized mitochondria moved into acidic vacuoles labeled by LysoTracker Red… 

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