The mitochondrial genome of safflower: Isolation and restriction fragment analysis of DNA from CMS and restorer lines

@article{Py1991TheMG,
  title={The mitochondrial genome of safflower: Isolation and restriction fragment analysis of DNA from CMS and restorer lines},
  author={Anik{\'o} P{\'a}y and Marvin Smith},
  journal={Molecular and Cellular Biochemistry},
  year={1991},
  volume={100},
  pages={151-157}
}
Mitochondria were isolated and purified from paired lines of safflower (Carthamus tinctorius L.) restorer and cytoplasmic male sterile plants using isopycnic gradient centrifugation in isoosmotic Percoll. Agarose gel electrophoresis of restriction endonuclease digested DNAs showed characteristic polymorphism. Restriction fragments representing about 75% of… CONTINUE READING