The mitochondrial genome is large and variable in a family of plants (Cucurbitaceae)

  title={The mitochondrial genome is large and variable in a family of plants (Cucurbitaceae)},
  author={Bernard Lloyd Ward and Robert S Anderson and Arnold J. Bendich},

Insights into the evolution of mitochondrial genome size from complete sequences of Citrullus lanatus and Cucurbita pepo (Cucurbitaceae).

The mitochondrial genomes of Citrullus lanatus and Cucurbita pepo are sequenced--the two smallest characterized cucurbit mitochondrial genomes--and their RNA editing content is determined and it is found that Cuculbita has a significantly higher synonymous substitution rate (and presumably mutation rate) than Citrulla but comparable levels of RNA editing.

Determination of the melon chloroplast and mitochondrial genome sequences reveals that the largest reported mitochondrial genome in plants contains a significant amount of DNA having a nuclear origin

The transfer of nuclear DNA to the melon mitochondrial genome and the high proportion of repetitive DNA appear to explain the size of the largest mitochondrial genome reported so far.

Origins and Recombination of the Bacterial-Sized Multichromosomal Mitochondrial Genome of Cucumber[C][W]

The 1685-kb mitochondrial genome of cucumber is reported, helping solve a 30-year mystery about the origins of its large size by showing that it mainly reflects the proliferation of dispersed repeats, expansions of existing introns, and the acquisition of sequences from diverse sources, including the cucumber nuclear and chloroplast genomes, viruses, and bacteria.

The linear 20 kb mitochondrial genome of Pandorina morum (Volvocaceae, Chlorophyta)

The Pandorina mitochondrial DNA shows sufficient similarity to the 16 kb mitochondrial genome of Chlamydomonas reinhardtii to cross-hybridize, and also hybridizes with a probe containing maize mitochondrial 18S rRNA genes.

Cucumber: a model angiosperm for mitochondrial transformation?

Cucumber possesses three unique attributes that make it a potential model system for mitochondrial transformation of a higher plant, and its mitochondria show paternal transmission, which could allow geneticists to use reverse genetics to study mitochondrial gene expression and to establish the efficacy of engineered mitochondrial genes for the genetic improvement of the mitochondrial genome.

Large size and complex structure of mitochondrial DNA in two nonflowering land plants

Because flowering plant mtDNAs are also large and contain dispersed, gene-containing, repeats, it appears that these features arose early in the evolution of land plants, or perhaps even in their green algal ancestors.

Rolling-circle replication of mitochondrial DNA in the higher plant Chenopodium album (L.)

Evidence is provided for a rolling-circle mode of replication in the mitochondria of higher plants by electron microscopy and a new approach based on the mapping of restriction fragments representing the identical 5' ends of the tails of sigma-like molecules.

Differential transmission of the Cucumis organellar genomes

This study identified RFLPs in the organellar genomes of melon, squash, and watermelon to establish organellar DNA transmission and identified the presence of a polymorphic cytoplasm when it represented at least 1% of the DNA sample.



DNA sequence organization in the pea genome.

It is concluded that the major class of single-copy sequences in pea DNA has a modal length of about 300 nucleotides, which is in good agreement with previous chemical and cytophotometric measurements.

Mitochondrial DNA from Oenothera berteriana: PURIFICATION AND PROPERTIES.

Unique to the mt-DNA from Oenothera berteriana, as compared to other higher plants, is the unusual high guanosine + cytosine content with 51% as determined by the buoyant density in CsCl of 1.710 grams/cubic centimeter and the melting point of 90 C.

Heterogeneous population of mitochondrial DNA molecules in higher plants

EcoRI specific cleavage is used to evidence the general occurrence of heterogeneous population of mt-DNA molecules within an higher plant and to detect clear differences between mt-DNAs extracted from normal and cytoplasmic male-sterile wheat.

Chloroplast DNA Sequence Homologies among Vascular Plants.

Determinations were made of the percentage of chloroplast DNA in total DNA extracts from different higher plants; this value remained relatively constant when compared with the large variation in the diploid genome size of the plants.

DNA from plant mitochondria.

It was found that all of these mitochondrial fractions contained DNA, the densities of which were identical, and DNA from nuclear fractions differed not only with each other but from the corresponding mitochondrial DNA.

Isolation and characterization of mitochondrial DNA from Chlamydomonas reinhardtii.

Thermal denaturation and renaturation studies have shown that mtDNA contains no extensive intramolecular heterogeneity nor significant base bias between the complementary polynucleotide chains and mtDNA renatures as a single homogeneous class with a kinetic complexity of 9.78 X 10(6) daltons.

Sequence arrangement in satellite DNA from the muskmelon.

The results are interpreted as an observation of a recently created family of tandemly repeating sequences whose members are beginning to be scattered throughout the genome.

Heterogeneity of Maize Cytoplasmic Genomes among Male-Sterile Cytoplasms.

The marked variation in mtDNA, with apparently less variation in ctDNA, represents circumstantial, but compelling, evidence that mtDNA may be involved in the male sterility and disease susceptibility traits in maize, and indicates a possible gradation of relatedness among male-sterile cytoplasms.

Recombinant DNA analysis indicates that the multiple chromosomes of maize mitochondria contain different sequences.

The variable abundance of different mitochondrial chromosomes is of special interest because it represents an unusual mechanism for the control of gene expression by regulation of gene copy number in a complex organelle genome.