The missing cone problem and low-pass distortion in optical serial sectioning microscopy

  title={The missing cone problem and low-pass distortion in optical serial sectioning microscopy},
  author={Fernando Mac{\'i}as-Garza and Alan Conrad Bovik and Kenneth R. Diller and Shanti J. Aggarwal and Jake K. Aggarwal},
  journal={ICASSP-88., International Conference on Acoustics, Speech, and Signal Processing},
  pages={890-893 vol.2}
In optical serial sectioning, the 3-D structure of a microscopic specimen is observed by incrementing the focusing plane of a light microscope through the specimen. If the depth of field of the microscope is infinitesimal, the image obtained from each focusing plane is an in-focus slice of the optical density of the specimen. The authors show that the finite aperture of any practical microscope inevitably results in the loss of a biconic region of frequencies in the 3-D Fourier spectrum of the… Expand
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