The minimal active domain of the mouse ras exchange factor CDC25Mm.

@article{Coccetti1995TheMA,
  title={The minimal active domain of the mouse ras exchange factor CDC25Mm.},
  author={Paola Coccetti and Isabella Mauri and Lilia Alberghina and Enzo Martegani and Andrea Parmeggiani},
  journal={Biochemical and biophysical research communications},
  year={1995},
  volume={206 1},
  pages={253-9}
}
The minimal active domain of the mouse CDC25Mm, a GDP/GTP exchange factor (GEF) active on H-ras protein, was determined by constructing several deletion mutants of the C-terminal domain of the protein. The functional activity of these fragments was analyzed for the ability to complement the yeast temperature sensitive mutation cdc25-1 and to catalyze the GDP/GTP exchange on Ras proteins in vitro. A C-terminal domain of 256 residues (CDC25Mm 1005-1260) was sufficient for full biological activity… CONTINUE READING