The minK potassium channel exists in functional and nonfunctional forms when expressed in the plasma membrane of Xenopus oocytes.

@article{Blumenthal1994TheMP,
  title={The minK potassium channel exists in functional and nonfunctional forms when expressed in the plasma membrane of Xenopus oocytes.},
  author={Edward M. Blumenthal and Leonard K Kaczmarek},
  journal={The Journal of neuroscience : the official journal of the Society for Neuroscience},
  year={1994},
  volume={14 5 Pt 2},
  pages={3097-105}
}
The minK protein induces a slowly activating voltage-dependent potassium current when expressed in Xenopus oocytes. In order to measure the levels of minK protein in the plasma membrane, we have modified the minK gene by inserting a 9 amino acid epitope into the N-terminal domain of the protein sequence. When intact live oocytes are injected with the modified minK RNA and subsequently incubated with an antibody to this epitope, specific binding is detected, indicating that the N-terminal domain… CONTINUE READING