The metabolism of poly(A)+ and poly(A)− hnRNA in cultured drosophila cells studied with a rapid uridine pulse-chase

@article{Levis1977TheMO,
  title={The metabolism of poly(A)+ and poly(A)− hnRNA in cultured drosophila cells studied with a rapid uridine pulse-chase},
  author={Robert W. Levis and Sheldon Penman},
  journal={Cell},
  year={1977},
  volume={11},
  pages={105-113}
}
Abstract When cultured Drosophila cells are pretreated with glucosamine and exposed to a pulse of incorporation of radioactive uridine, their labeling can be halted almost instantaneously by the addition of unlabeled uridine. Using this pulse-chase technique, we have investigated the decay of hnRNA and the formation of mRNA. The poly(A) + and poly(A) − hnRNAs behave as different populations. The poly(A) hnRNA constitutes 80% of the pulse-labeled material and decays with an initial half-life of… Expand
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