The metabolism and biological activity of esterified vitamin D in the rat.

  title={The metabolism and biological activity of esterified vitamin D in the rat.},
  author={David R. Fraser and E. Kod{\'i}{\vc}ek},
  journal={The British journal of nutrition},
  volume={23 1},
Materials. Radioactive vitamin D was obtained by saponification of [ ~-~H]cholecalciferol 3,s-dinitrobenzoate and subsequent purification of the product as described by Wilson, Lawson & Kodicek (1967). Unlabelled crystalline cholecalciferol (KochLight Laboratories Ltd, Colnbrook, Bucks.) was used to dilute the labelled form to appropriate specific activity. The palmitic acid ester of [ ~-~H]cholecalciferol (specific radioactivity 141 mc/m-mole) was prepared (Fraser & Kodicek, 1968a) and was… 
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Recent advances in vitamin D metabolism
It is shown that vitamin D, added in vivo, increased the uptake of orotic acid into nuclear and total RNA and increased the template activity of DNA (Norman, 1966; Stohs, Zull and DeLuca, 1967; Lawson et al 1969c; Hallick and De Luca, 1969).
Synthesis and biological activity of 1α-hydroxyvitamin D3 1-butyrate
14. 15. V. V. Gatsura, Methods for Primary Pharmacological Study of Biologically Active Substances [in Russian], Moscow (1974), p. 27. P M. Gocherkin, V. I. Linenko, A. A. Tkachenko, et al.,
The Metabolic Fate of (26,27) 3H‐25‐Hydroxyvitamin D3 in Normal, Uremic and Rachitic Rats
The results suggest that a condition of uremia “normalizes” is some way the enzymatic disturbances provoked by a state of severe rickets.
Metabolism of 25-Hydroxycholecalciferol in Nephrectomized Rats
The results suggest that a disorder of vitamin D metabolism is not very likely to be the cause of the development of renal bone disease and that the kidney does not play an important role in the metabolism of Vitamin D.
Die Cytidinmonophosphat-N-acetylneuraminat-Synthetase normaler und leukämischer Leukocyten des Menschen
The cytidine-5′-monophosphate-N-acetylneura-minate-synthetase shows a normal or even increased activity in chronic lymphatic leukemic lymphocytes, although there is a marked decrease on the surface of these cells in the neuraminic acid, which is part of the PHA receptor.


Investigations on vitamin D esters synthesized rats. Detection and identification.
The proportion of radioactivity in the saturated fatty acid esters was higher in kidney than in liver, and the same percentage of tissue vitamin D in the esterified form was found at each of two dosages of vitamin D.
Investigations on vitamin D esters synthesized in rats. Turnover and sites of synthesis.
The tissue contents of vitamin D alcohol and ester were estimated in rats 5, 10, 24, 48 and 72hr after peroral administration of [1-(3)H]cholecalciferol, and it is suggested that ester found in liver and kidney could have been transported to these sites in the blood.
Enzyme studies on the esterification of vitamin D in rat tissues.
It is concluded that cholesterol-esterifying enzymes esterify vitamin D in vivo during absorption from the small intestine and while it is transported in blood.
An investigation of the metabolism of vitamin D3 and of its biologically active forms in rat kidney and intestinal mucosa was carried out utilizing radiochemically pure H3-vitamin D3, and at least three other chloroform-soluble radioactive compounds were detected, all of which showed partial vitamin D activity.
The intracellular distribution of [1-3H]cholecalciferol in the intestine of vitamin D-deficient and -supplemented rats.
Experiments conducted in vitro have shown that all the intracellular particles combine with the vitamin either when dissolved in ethanol or bound to albumin, however, such an uptake does not account for the high concentration of radioactivity found in vivo in the fraction containing nuclei and brush border, nor for the tightly bound vitamin in the microsomal fraction.
Relative activities of free and esterified vitamin D.
Halibut liver oils showed anomalous behaviour in this respect, saponification resulting in either increased or unaltered activity, depending on the previous history of the material.
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